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E results indicate that secreted SK is a potent contact system activator, exhibiting its function independently from a contact surface. The chromogenic substrate S-2302 is specific for PK/FXIIa but is also sensitive to plasmin. We therefore measured the PK/FXIIa activity after addition of chloromethyl-ketone D-Val-Phe-Lys, which is an efficient inhibitor of plasmin (30) and the SK-plasminogen comp

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